Movie S2. VTLM of CD19-specific CAR+ T cells and Aspergillus germling. CD19-specific CAR+ T cells were coincubated with GFP+ Aspergillus (AF293) swollen conidia (E:T ratio of 2:1), and one germling (target, T) was imaged continuously for 24 h by VTLM in phenol red-free medium. The movie shows no apparent inhibition of growth of GFP+AF293 germinating conidia (green fluorescence) by D-CAR+ T cells.
Pappanaicken R. Kumaresan, Pallavi R. Manuri, Nathaniel D. Albert, Sourindra Maiti, Harjeet Singh, Tiejuan Mi, Jason Roszik, Brian Rabinovich, Simon Olivares, Janani Krishnamurthy, Ling Zhang, Amer M. Najjar, M. Helen Huls, Dean A. Lee, Richard E. Champlin, Dimitrios P. Kontoyiannis, and Laurence J. N. Cooper
PNAS. 2014. 111:10660-10665 DOI: 10.1073/pnas.1312789111