Video 1

Movie S1. Live imaging movie of Dronpa, rsKame, and mEos2. EpH4 cells were transfected with Dronpa, rsKame, or mEos2 targeted to the outer mitochondrial membrane. Dronpa- and rsKame-labeled cells were illuminated with 488-nm light only, whereas mEos2-labeled cells were illuminated simultaneously by 488 nm and 405 nm. Single-molecule fluorescence events are more clearly distinguishable for rsKame than Dronpa owing to the slower 488 nm-induced photo-switching of rsKame. The video shows a small fraction of the whole cell’s mitochondrial population under illumination.