Video 2

Movie S2.Comparison of mGeosM and rsKame. HeLa cells expressing mGeosM and rsKame targeted to the OMM were first illuminated by 5.9 W/mm2 of 488 nm only. Both fluorescent proteins exhibit initial fluorescence that quickly photobleaches. At 4:10, 160 mW/mm2 of 405 nm were added to photoactivate all of the remaining molecules, and samples were imaged until fully photobleached.

Optimized two-color super resolution imaging of Drp1 during mitochondrial fission with a slow-switching Dronpa variant

Alyssa B. Rosenbloom, Sang-Hyuk Lee, Milton To, Antony Lee, Jae Yen Shin, and Carlos Bustamante

PNAS. 2014. 111:13093-13098 DOI: 10.1073/pnas.1320044111