Movie S2.Comparison of mGeosM and rsKame. HeLa cells expressing mGeosM and rsKame targeted to the OMM were first illuminated by 5.9 W/mm2 of 488 nm only. Both fluorescent proteins exhibit initial fluorescence that quickly photobleaches. At 4:10, 160 mW/mm2 of 405 nm were added to photoactivate all of the remaining molecules, and samples were imaged until fully photobleached.