Video 1

Movie S1. T cells arrest during early infiltration and regain motility with increased infiltration. Movie of the data represented in Fig. 1A. CD2-dsRed.OT-I CD8+ T cells or a combination of 10% CD2-dsRed.OT-I CD8+ T cells and 90% OT-I CD8+ T cells were transferred into RIP-mOva recipient mice. Islets were isolated 4–7 d following T-cell transfer and immobilized in low-melting agarose. Isolated islets were imaged by time-lapse two-photon microscopy. Representative maximum intensity projection images of T-cell (red) motility in islets with light, mid, and heavy T-cell infiltration. All infiltrating OT-I T cells are visualized in the islet with light infiltration whereas only 10% of infiltrating OT-I T cells are visualized in the islets with mid and heavy infiltration. The yellow line indicates the islet border. (Scale bars: 30 μm.) Time stamp, min:sec.