Time-lapse imaging of SDP1 in hypocotyl of vps29 seedlings at 4 DAG. vps29 mutant seedlings expressing GFP–SDP1 were grown on MS medium with 1% (wt/vol) sucrose. At 4 DAG, seedlings were treated with Nile Red and imaged with a spinning disk confocal microscope (Leica) during 15 min. Green labeling corresponds to GFP–SDP1 and red labeling to OBs. (Scale bars: 20 µm.) Along with Movies S1, S2, and S4, this movie relates to Fig. 4.