Video 5

Movie S5. Optineurin recruitment to the surface of individual parkin-labeled damaged mitochondria. Live cell confocal video microscopy of a HeLa cell expressing mCherry-Optn, YFP-parkin, and BFP-mito after ∼40 min of CCCP treatment. (Lower Right) Optineurin begins as a single puncta on the side of a parkin-labeled mitochondria and forms a complete ring covering the mitochondrial surface. (Upper Left) Optineurin is gradually recruited to a parkin-labeled mitochondria until it forms a complete ring around the mitochondria. Video corresponds to Fig. 2I. Mitochondrial signal bleaches over time. Images were captured at 1 frame per 3 s and played back at 10 frames per second. (Scale bar, 1 µm.)