Movie S1. Focal axonal degeneration (FAD) progression in the ventral spinal cord. Thy1-YFP+ axons (yellow) are visualized under two-photon (2P) excitation in the explanted ventral spinal cord 1 wk following JHMV infection. Axons do not exhibit FAD morphology in the noninfected spinal cord (Left). FAD is observed in the JHMV-infected spinal cord (Right) with areas of active pathology highlighted in red. Movie duration = 31:06 (min:s). (Scale bar, 20 μm.)