Video 8

Movie S8. Time-lapse movie of the growth of the nanowire later observed by OmcA-specific immunofluorescence in Fig. 3D. Red fluorescence is the result of membrane staining by FM 4-64FX. The specific nanowire seen here was immunofluorescently imaged again (Fig. 3D) after reacting with the primary OmcA-specific antibody and FITC-conjugated secondary antibody. This movie shows that the target structure in Fig. 3D was in fact a nanowire that was produced under electron–acceptor-limiting conditions inside the perfusion chamber. The interval between two consecutive frames is 5 min, but is reduced to 200 ms in the movie. (Scale bar: 5 µm.)