Video 1

Movie S1. Complete virus disintegration. Full virus disintegration allowing outward diffusion of all HA–CR8020 complexes into the DSPE–PEG-supported bilayer shortly after full fusion. Labeling of the viral lumen by SRB was sufficient in this virus to allow visualization of the SRB molecules as they diffuse in the space between the glass coverslip and the tethered bilayer after full fusion. SRB fluorescence from the viral lumen is colored in magenta, CR8020 fluorescence is colored in green, overlap between the two is seen as white. (Scale bars, 2 μm.) The full fusion event is indicated with a magenta arrow and HA–CR8020 diffusion is indicated by green arrows.