Video 2

Movie S2. Partial virus disintegration. Partial virus disintegration allowing a portion of the HA–CR8020 complexes to diffuse outward into the DSPE–PEG-supported bilayer shortly after full fusion. The portion of HA–CR8020 observed to break off from the main virus particle is seen to itself disintegrate, with smaller HA–CR8020 clusters diffusing outward. SRB fluorescence from the viral lumen is colored in magenta, CR8020 fluorescence is colored in green, overlap between the two is seen as white. (Scale bars, 2 μm.) The full fusion event is indicated with a magenta arrow and the main HA–CR8020 diffusion event is indicated by green arrows.