Video 3

Movie S3. Long diffusion of small HA-CR8020 cluster. Release of a small cluster of HA–CR8020 complexes into the DSPE–PEG-supported bilayer after full fusion occurs. The cluster stays together for a substantial amount of time and undergoes long-range, 2D diffusion. SRB fluorescence from the viral lumen is colored in magenta, CR8020 fluorescence is colored in green, overlap between the two is seen as white. (Scale bars, 2 μm.) The full fusion event is indicated with a magenta arrow and the initial portion of the HA–CR8020 diffusion trajectory is indicated by green arrows.