Video 3

Movie S3. Tubules emerge from maturing E. coli-containing phagosomes hours after phagocytosis in DCs. This movie illustrates tubule formation from phagosomes after WT DCs were infected with E. coli-eGFP and chased for 2 h as in Fig. 1D. (Left) eGFP fluorescence. (Right) Overlay of fluorescence over the phase-contrast image. The time scale in seconds is indicated at the lower left.

TLR-dependent phagosome tubulation in dendritic cells promotes phagosome cross-talk to optimize MHC-II antigen presentation

Adriana R. Mantegazza, Allison L. Zajac, Alison Twelvetrees, Erika L. F. Holzbaur, Sebastián Amigorena, and Michael S. Marks

PNAS. 2014. DOI: 10.1073/pnas.1412998111