Video 4

Movie S4. Phagosomal tubulation in DCs requires TLR4-MyD88 signaling. This movie shows phagotubule formation (or the absence thereof) at 2.5 h after phagocytosis of LPS/OVA-TxR beads in untreated WT BMDCs, untreated Myd88−/− BMDCs, or WT BMDCs pretreated with TBK-1 inhibitor or IRAK-1/4 inhibitor (IRAK-1/4 inhibitor PT), or treated with IRAK-1/4 inhibitor at pulse (IRAK-1/4 at pse) or at chase as indicated. The time scale in seconds is indicated at the lower left.

TLR-dependent phagosome tubulation in dendritic cells promotes phagosome cross-talk to optimize MHC-II antigen presentation

Adriana R. Mantegazza, Allison L. Zajac, Alison Twelvetrees, Erika L. F. Holzbaur, Sebastián Amigorena, and Michael S. Marks

PNAS. 2014. DOI: 10.1073/pnas.1412998111