Video 6

Movie S6. Phagosomal tubulation in DCs requires an intact cytoskeleton, but not phagolysosome formation. This movie shows phagotubule formation (or absence thereof) at 2.5 h after phagocytosis of LPS/OVA-TxR beads by WT BMDCs pretreated with DMSO (vehicle), bafilomycin A1, or 3-MA, treated with 3-MA at pulse (3-MA at pse) or treated at chase with 3-MA, nocodazole, or latrunculin B as indicated. The time scale in seconds is indicated at the lower left.

TLR-dependent phagosome tubulation in dendritic cells promotes phagosome cross-talk to optimize MHC-II antigen presentation

Adriana R. Mantegazza, Allison L. Zajac, Alison Twelvetrees, Erika L. F. Holzbaur, Sebastián Amigorena, and Michael S. Marks

PNAS. 2014. DOI: 10.1073/pnas.1412998111