Movie S8. Phagotubules do not fuse with the plasma membrane. This movie shows WT BMDCs at 2.5 h after phagocytosis of LPS/OVA-TxR beads captured by epifluorescence (green, Left) and TIRF microscopy (red, Center), showing the extension of a phagotubule (red) within the TIRF zone approaching and retracting from, but not fusing with, the plasma membrane. (Right) Merged image. The time scale in seconds is indicated at the lower left.