Movie S1.

HAP labeling was mainly observed on the surface of spherules. This was not the consequence of poor dye penetration, because the labeling was associated with the surface even on spherules that were cut on paraffin sections.

Identification of hydroxyapatite spherules provides new insight into subretinal pigment epithelial deposit formation in the aging eye

Richard B. Thompson, Valentina Reffatto, Jacob G. Bundy, Elod Kortvely, Jane M. Flinn, Antonio Lanzirotti, Emrys A. Jones, David S. McPhail, Sarah Fearn, Karsten Boldt, Marius Ueffing, Savanjeet Guy Singh Ratu, Laurenz Pauleikhoff, Alan C. Bird, and Imre Lengyel

PNAS. 2015. 112:1565-1570 DOI: 10.1073/pnas.1413347112