HAP labeling was mainly observed on the surface of spherules. This was not the consequence of poor dye penetration, because the labeling was associated with the surface even on spherules that were cut on paraffin sections.
Richard B. Thompson, Valentina Reffatto, Jacob G. Bundy, Elod Kortvely, Jane M. Flinn, Antonio Lanzirotti, Emrys A. Jones, David S. McPhail, Sarah Fearn, Karsten Boldt, Marius Ueffing, Savanjeet Guy Singh Ratu, Laurenz Pauleikhoff, Alan C. Bird, and Imre Lengyel
PNAS. 2015. 112:1565-1570 DOI: 10.1073/pnas.1413347112