Video 1

Movie S1. M10 cells stably expressing Langerin-eYFP acquired sequentially using TIRF and WF illumination. On the Left are original data with the WF in the red channel and TIRF in the green channel; Middle, the Langerin-associated vesicles are tracked in the WF image sequence; and Right, the docking events are detected in the TIRF plane.

Fast high-resolution 3D total internal reflection fluorescence microscopy by incidence angle scanning and azimuthal averaging

Jérôme Boulanger, Charles Gueudry, Daniel Münch, Bertrand Cinquin, Perrine Paul-Gilloteaux, Sabine Bardin, Christophe Guérin, Fabrice Senger, Laurent Blanchoin, and Jean Salamero

PNAS. 2014. 111:17164-17169 DOI: 10.1073/pnas.1414106111