Video 4

Movie S4. Volume rendering with four viewing angles of a time sequence of reconstructed multiangle TIRF image stack showing a RPE1 cell expressing LifeAct-mCherry. The total depth of the volume is 500 nm. The exposure time was 50 ms per image, and stacks have been acquired every 5 s. The acquired image stacks are composed of 11 planes corresponding to 11 angles.