Movie S1.

Three-dimensional model of protein distributions for SYCP3 (red), SYCP2 (green), the SYCP1 C terminus (cyan), and the SYCP1 N terminus (yellow) is illustrated. Solid cables show averaged protein positions with the width of the SD of the averaged position (6.07 nm for SYCP3, 6.39 nm for SYCP2, 2.58 nm for the SYCP1 C terminus, and 4.84 nm for the SYCP1 N terminus). Cloudy dots indicate measured FWHM of single-molecule localization distributions (55.8 nm for SYCP3, 51.1 nm for SYCP2, 45.2 nm for the SYCP1 C terminus, and 39.8 nm for the SYCP1 N terminus).