Video 1

Movie S1. CquiOR136 knockdown mosquitoes are not repelled by DEET. Still images of the arena and an assay with WT mosquitoes are shown at the beginning of the movie. Then, movie images captured with a night vision infrared camera in the same manner as described for Fig. 2 are shown initially at regular and then at 2× speed. The movie shows very active females landing on both sides of the arena starting at the onset of the experiment. Intermittent red light inspections help visualize mosquitoes feeding. CO2 at 50 mL/min was delivered at both sides of the arena, just above Dudley bubbling tubes, whereas defibrinated sheep blood was loaded into cotton rolls and presented as a feeding reward between CO2 source and warm black tubes. Each Dudley tube was surrounded by a paper ring, loaded either with DEET or solvent only.

Mosquito odorant receptor for DEET and methyl jasmonate

Pingxi Xu, Young-Moo Choo, Alyssa De La Rosa, and Walter S. Leal

PNAS. 2014. DOI: 10.1073/pnas.1417244111