Movie S4.

Dual-color luminescence imaging of Ca2+ indicators. Time-lapse dual-color luminescent imaging of Mito-CNL(Ca2+) and ONL(Ca2+)-H2B in HeLa cells. Images of each color were acquired simultaneously using W-View equipped with 550-nm long- and short-path filters for 7.5 min at the rate of 1 frame per 1 s; 20 μM histamine was added at 45 s after the start of imaging. Playback speed is 30-fold. (Scale bar: 10 μm.)