Movie S2.

Movement of single actin-mEos2 molecules in a growth cone migrating on an Ncad-Fc–coated micropatterned substrate. The still image of green-emitting actin-Eos2 is coded in blue, micropatterns are delineated by white dashed circles, and photoconverted actin-mEos2 molecules appear as red spots. Note that actin-mEos2 molecules move steadily rearward and slow down when they reach micropatterns, revealing interactions with N-cadherin adhesions.