PI3K therapy stimulates membrane ruffle dynamics. PC3 cells treated with vehicle (Veh) or the indicated PI3K inhibitors for 48 h were analyzed by time-lapse video microscopy. Four discrete regions per cell (arrows) were followed over time (0.5 s per frame, 500 frames) to derive stroboscopic images. Magnification, 40×.
M. Cecilia Caino, Jagadish C. Ghosh, Young Chan Chae, Valentina Vaira, Dayana B. Rivadeneira, Alice Faversani, Paolo Rampini, Andrew V. Kossenkov, Katherine M. Aird, Rugang Zhang, Marie R. Webster, Ashani T. Weeraratna, Silvano Bosari, Lucia R. Languino, and Dario C. Altieri
PNAS. 2015. DOI: 10.1073/pnas.1500722112