Movie S4.

A human glioblastoma cell undergoing apoptosis. A human glioblastoma LN229 cell stably expressing iCasper was imaged in the GFP and IFP channel upon addition of 1 μM staurosporin. Images were taken every 10 min. The green and infrared fluorescence images were merged. The movie was taken under a 40× objective lens. The cell has a diameter of about 20 nm.

Rationally designed fluorogenic protease reporter visualizes spatiotemporal dynamics of apoptosis in vivo

Tsz-Leung To, Beverly J. Piggott, Kalpana Makhijani, Dan Yu, Yuh Nung Jan, and Xiaokun Shu

PNAS. 2015. DOI: 10.1073/pnas.1502857112