Movie S5.

Neurons in a small region of VNC undergoing apoptosis. A small region of the VNC in an intact Drosophila embryo was imaged every 2 min. The neurons expressed both CD8-GFP and iCasper. The cell membrane was marked by CD8-GFP. The green and infrared fluorescence images were merged. The movie was taken under a 40× objective lens. The cells have a diameter of about 10 nm.

Rationally designed fluorogenic protease reporter visualizes spatiotemporal dynamics of apoptosis in vivo

Tsz-Leung To, Beverly J. Piggott, Kalpana Makhijani, Dan Yu, Yuh Nung Jan, and Xiaokun Shu

PNAS. 2015. DOI: 10.1073/pnas.1502857112