Movie S2.

Movie showing fluorescence intensity of (Upper) the Ca2+ indicator G-CaMP (a fusion of green fluorescent protein, calmodulin, and M13, a peptide sequence from myosin light chain kinase) and (Lower) red fluorescent protein (RFP)-Lifeact on force application. The color code is in fire scale and was prepared using ImageJ. The movie is 17 times faster than real time. This movie is related to Fig. 2A.