Movie S3.

Movie showing fluorescence intensity of (Upper) the Ca2+ indicator G-CaMP (a fusion of green fluorescent protein, calmodulin, and M13, a peptide sequence from myosin light chain kinase) and (Lower) red fluorescent protein (RFP)-Lifeact on addition of calcium ionophore A23187. The color code is in fire scale and was prepared using ImageJ. The movie is 30 times faster than real time. This movie is related to Fig. 3A.

Mechanical stimulation induces formin-dependent assembly of a perinuclear actin rim

Xiaowei Shao, Qingsen Li, Alex Mogilner, Alexander D. Bershadsky, and G. V. Shivashankar

PNAS. 2015. DOI: 10.1073/pnas.1504837112