Dynamics of INF2-GFP and mApple-paxillin at focal adhesions and the leading edge of MEF. MEF expressing human INF2-GFP lacking the membrane-targeting CAAX domain (Left) and mApple-paxillin (Right) were cultured on fibronectin-coated coverslips and imaged by TIRF microscopy. Time indicates minutes:seconds. (Scale bar, 10 µm.)