Movie S1.

Superimposed onto a DIC image of three cells of S. aureus JGL237 (which contains an EzrA–GFP fusion) grown in the absence of inducer and therefore partly depleted for PlsY, there is a fluorescence microscopy image of the same cells revealing an abnormal distribution of EzrA. Although EzrA normally forms a ring at the division site, in the absence of PlsY, it seems unable to complete a faithful ring (like the example shown), resulting in a spiral shape.