Movie S3.

Immunofluorescence microscopy Z-stacks of a S. aureus LC102 revealing the regular distribution of PlsY in its membrane. PlsY was labeled with α-731 primary antibody and with A-11008 Alexa Fluor 488 as a secondary antibody. A cell within the microscopic field (cell 1) has been magnified to provide a more detailed view of the patterned distribution of PlsY. Frame 98.83 × 98.83 μm.