Movie S1.

Explant imaging of the retina (arrowhead, RGC cell body) of Thy1-mitoCFP-S mice shows axonal transport of mitochondria in an RGC axon (asterisk). The ONH is on the right side in this movie. Note that this explant imaging was conducted near the ONH where the density of RGC axons is high, because axonal transport of mitochondria in the retinal explants was rarely observed even though the retinal explants were maintained under conditions optimized for long-term retinal cultures. Cutting of the optic nerves (RGC axons) to prepare retinal explants induced excessive mitochondrial fragmentation in the RGC axons. Time is indicated as min:s.