Movie S4.

Three-dimensional rendering and z-stack images of the hippocampus region stained for SYTO 16, lectin, and anti-histone H3 antibody. Three-dimensional rendering of the hippocampus region showing uniform and complete nuclear counterstaining, lectin staining, and immunostaining achieved with stochastic electrotransport. SYTO 16, green; lectin, red; anti-histone H3, white. Approximately 2 × 2 × 5 mm3 volume was imaged using a confocal microscope in 2.5 d (10× CLARITY-optimized objective; N.A., 0.6; working distance, 8.0 mm; z-step size, 20 μm). Note that the red dots near the surface were caused by spectral bleed-through from SYTO 16. This was corrected in Fig. 6E.