Movie S5.

Three-dimensional rendering of a mouse brain hemisphere stained with lectin. A CLARITY-processed adult mouse brain hemisphere was stained with tomato lectin to label the vasculature using stochastic electrotransport for 10 h. Note the uniform and complete labeling of the vasculature. Approximately 10 × 7 × 2 mm3 volume was imaged using a Zeiss Z-1 light-sheet microscope within 1.5 h (20× CLARITY-optimized objective; N.A., 1.0; working distance, 5.1 mm; z-step size, 4 μm).