Movie S2.

Time-lapse confocal imaging and FRAP of the SNCA−/− neuron expressing mEOS2–α-Syn (shown in Fig. 3F). An individual inclusion (red circle) was photobleached using a 488-nm laser, and fluorescence recovery from diffusing soluble protein was monitored over time. (Scale bar: 2 µm.)

Induction of de novo α-synuclein fibrillization in a neuronal model for Parkinson’s disease

Mohamed-Bilal Fares, Bohumil Maco, Abid Oueslati, Edward Rockenstein, Natalia Ninkina, Vladimir L. Buchman, Eliezer Masliah, and Hilal A. Lashuel

PNAS. 2016. 113:E912-E921 DOI: 10.1073/pnas.1512876113