In vivo label-free SRS imaging cell division dynamics in cancer. Synchronized HeLa cells were injected into a xenograft mouse model with a skinfold chamber. Imaging was taken 26 h after the injection. Cells in mitotic phase that landed in the subskin tissue microenvironment (protein contrast in blue) were targeted and imaged. Chromosomal dynamics were captured based on DNA contrast (magenta). The video was composed of 12 frames acquired with a 3-min interval. For experimental details, refer to Materials and Methods and Figs. S3 B and C.
Fa-Ke Lu, Srinjan Basu, Vivien Igras, Mai P. Hoang, Minbiao Ji, Dan Fu, Gary R. Holtom, Victor A. Neel, Christian W. Freudiger, David E. Fisher, and X. Sunney Xie
PNAS. 2015. DOI: 10.1073/pnas.1515121112