Movie S2.

Auxin treatment mainly leads to constricted and not to fragmented vacuoles. The 3D vacuole reconstruction after auxin treatment (250 nM NAA, 20 h) of micrographs was recorded by SEM using Amira software.

Actin-dependent vacuolar occupancy of the cell determines auxin-induced growth repression

David Scheuring, Christian Löfke, Falco Krüger, Maike Kittelmann, Ahmed Eisa, Louise Hughes, Richard S. Smith, Chris Hawes, Karin Schumacher, and Jürgen Kleine-Vehn

PNAS. 2015. 113:452-457 DOI: 10.1073/pnas.1517445113