Live-cell imaging of auxin-treated vacuoles shows multiple constrictions. The 3D reconstruction shows meristematic vacuoles stained by the luminal dye BCECF-AM and PI (cell wall) after auxin treatment (250 nM NAA, 20 h).
David Scheuring, Christian Löfke, Falco Krüger, Maike Kittelmann, Ahmed Eisa, Louise Hughes, Richard S. Smith, Chris Hawes, Karin Schumacher, and Jürgen Kleine-Vehn
PNAS. 2015. 113:452-457 DOI: 10.1073/pnas.1517445113