Movie S6.

Unwinding of DNA by stable RecQ oligomers. The elapsed time is indicated in hours:minutes:seconds. DNA unwinding was initiated by using the microfluidic injection system (Fig. S7) with the loop containing 70 nM RecQ, 60 nM AF488SSB, 1 mM ATP, 1 mM Mg(OAc)2, and 75 nM Sytox Orange in SM buffer. This solution is pumped into and fills the flow cell in Fig. 4A at 39 s into the movie. Subsequently, 50 µL of a solution containing 60 nM AF488SSB, 1 mM ATP, 1 mM Mg(OAc)2, and 75 nM Sytox Orange is pumped into the flow cell to remove the free RecQ, starting at 1:03 into the movie and ending at 1:27. The forks are observed to continue to unwind after removing free RecQ.