Movie S2.

Time-elapse fluorescence imaging of the response of three individual HeLa cells patterned on H-00 islands (32 × 32 µm) coated with fibronectin at different standoff distances (Sd) produced by tandem bubble-induced jetting flow. White arrows indicate jetting direction. LifeAct-GFP is used to label F-actin (green) and PI is used to indicate membrane poration after the treatment.