Movie S1.

Accumulation of microglial cell processes (EGFP; green) after focal laser injury at cerebral tissue away from capillaries (Texas Red-dextran; red) in CX3CR1+/EGFP/P2RY12+/+ mice. Cross sign indicates the point of the injury, devoid of blood vessels and microglial processes. The movie was taken immediately after the injury with 12 s per frame for about 20 min in cortical layer 2. Scale (µm) and time (min:s) are indicated in the movie.

Purinergic receptor P2RY12-dependent microglial closure of the injured blood–brain barrier

Nanhong Lou, Takahiro Takano, Yong Pei, Anna L. Xavier, Steven A. Goldman, and Maiken Nedergaard

PNAS. 2016. 113:1074-1079 DOI: 10.1073/pnas.1520398113