Movie S6.

Accumulation of microglial cell processes (EGFP; green) after a focal laser injury at a capillary (Texas Red-dextran; red) in cerebral cortex of CX3CR1+/EGFP/P2RY12+/+ mice treated with clopidogrel. Cross sign indicates the point of the injury. The movie was taken immediately after the injury with 10 s per frame for about 30 min in cortical layer 2. Scale (µm) and time (min:s) are indicated in the movie.

Purinergic receptor P2RY12-dependent microglial closure of the injured blood–brain barrier

Nanhong Lou, Takahiro Takano, Yong Pei, Anna L. Xavier, Steven A. Goldman, and Maiken Nedergaard

PNAS. 2016. 113:1074-1079 DOI: 10.1073/pnas.1520398113