Movie S1.

Movie of HH stage 10–11 (E1.5–E2) chick hearts preonset and postonset of beating. Prebeating hearts exhibit “shivering” behavior of uncoordinated cardiomyocyte contractions. Postbeating hearts exhibit fully coordinated beats. Movie plays at 3× real time. Micropipette in view has an outer diameter of ∼50 μm.

Mechanical signaling coordinates the embryonic heartbeat

Kevin K. Chiou, Jason W. Rocks, Christina Yingxian Chen, Sangkyun Cho, Koen E. Merkus, Anjali Rajaratnam, Patrick Robison, Manorama Tewari, Kenneth Vogel, Stephanie F. Majkut, Benjamin L. Prosser, Dennis E. Discher, and Andrea J. Liu

PNAS. 2016. 113:8939-8944 DOI: 10.1073/pnas.1520428113