Movie S1.

Time-lapse, live-cell fluorescence microscopy of anti-LINGO1 antibody incubated with Ewing sarcoma A673 cells. Time-lapse image of Alexa Fluor 488-labeled anti-LINGO1 antibody bound to A673 cells overlaid with LysoTracker Deep Red signals. The colocalization is indicated by yellow. Cells were incubated at 37 °C in DMEM with 10% (vol/vol) FBS, and images were collected at 25-s intervals for 1 h using the DeltaVision Elite Imaging System.