Movie S2.

Engineered chimeric motor rescues MT sliding in the absence of endogenous KHC. Representative photoconversion time-lapse movies of S2 cells transfected with tdEOS-tubulin and different chimeric motor constructs (UncLZ, UncLZ-Ktail, or UncLZ-KtailmutA). Endogenous KHC was depleted in each condition by dsRNA against the 3′-UTR. A chimeric motor that contains the motor domain of kinesin-3/Unc104 fused to the extreme C terminus of KHC (UncLZ-Ktail) rescued MT sliding, whereas the control construct (UncLZ) or KHCmutA alanine substitution in this construct (UncLZ-KtailmutA) prevented rescue. (Scale bar, 5 μm.)