Movie S3.

KHCmutA maintains normal peroxisome transport in S2 cells. Representative time-lapse movies of S2 cells transfected with peroxisome targeting sequence, GFP-SKL, and wild-type KHC (Left) or KHCmutA (Right). Endogenous KHC was depleted in both cells by dsRNA against the 3′-UTR. Note that both KHC and KHCmutA can transport peroxisomes. (Scale bar, 5 μm.)