KHCmutA maintains normal mitochondria transport in S2 cells. Representative time-lapse movies of S2 cells transfected with wild-type KHC (Left) or KHCmutA (Right). Endogenous KHC was depleted in both cells by dsRNA against the 3′-UTR. Mitochondria were imaged after staining cells with MitoTracker Red. Note that both KHC and KHCmutA can transport mitochondria. (Scale bar, 5 μm.)