Intracellular neutrophil handling of A. baumannii is within low-pH conditions in phagolysosomes. Time-lapse confocal z-stack imaging of the somatic muscle after infection with pHrodo-stained A. baumannii (ATCC 17978) by using zebrafish embryos with green fluorescent neutrophils [Tg(mpx:EGFP)i114]. Maximum projection of z-stacks showing phagolysosome formation in green neutrophils.
Md Saruar Bhuiyan, Felix Ellett, Gerald L. Murray, Xenia Kostoulias, Gustavo M. Cerqueira, Keith E. Schulze, Mohd Hafidz Mahamad Maifiah, Jian Li, Darren J. Creek, Graham J. Lieschke, and Anton Y. Peleg
PNAS. 2016. 113:9599-9604 DOI: 10.1073/pnas.1523116113