Movie S4.

OPTN and TBK1 are recruited to depolarized mitochondria after CCCP treatment. The live-cell confocal movie shows the recruitment of GFP-OPTN (green) and SNAP-TBK1 (red) to Mito-DsRed2 (blue) in a Parkin-expressing HeLa cell treated with 20 μm CCCP. Images were captured at one frame every 30 s. The time stamp indicates time after CCCP treatment (minutes:seconds). (Scale bar: 5 μm.)