Movie S5.

Cutaneous axon regeneration in 3-dpf vehicle control larva. Caudal fin amputation in a larva treated for 3 h with 0.5% DMSO/Ringers induces axon regeneration. Shown is a 12-h time-lapse sequence with individually recorded z stacks, which were projected into a single plane, every 30 min. One cutaneous axon of a Rohon-beard neuron with multiple branches is fluorescently labeled following transient injection of CREST3:Gal4_UAS:GFP into one-cell stage embryos. The second sequence shows regenerating axons that were manually traced in Fiji.