Impaired axon regeneration is partially rescued with DB04760. Treatment for 3 h with 22 µM paclitaxel and 10 µM DB04760 partially rescues impaired axon regeneration. Shown is a time-lapse sequence with individually recorded z stacks, which were projected into a single plane, every 30 min. One cutaneous axon of a Rohon-beard neuron with multiple branches is fluorescently labeled following transient injection of CREST3:Gal4_UAS:GFP into one-cell stage embryos. The second sequence shows regenerating axons that were manually traced in Fiji.
Thomas S. Lisse, Leah J. Middleton, Adriana D. Pellegrini, Paige B. Martin, Emily L. Spaulding, Olivia Lopes, Elizabeth A. Brochu, Erin V. Carter, Ashley Waldron, and Sandra Rieger
PNAS. 2016. DOI: 10.1073/pnas.1525096113